Details

Autor(en) / Beteiligte

• Zhao, Na
• Zhu, Meichen
• Liu, Qianqian
• Shen, Yanmei
• Duan, Shipeng
• Zhu, Lirong
• Yang, Jinkui

Titel

AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism in Arthrobotrys oligospora

Ist Teil von

• Journal of fungi (Basel), 2024-02, Vol.10 (2), p.110

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2024

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Prdx2 is a peroxiredoxin (Prx) family protein that protects cells from attack via reactive oxygen species (ROS), and it has an important role in improving the resistance and scavenging capacity of ROS in fungi. is a widespread nematode-trapping fungus that can produce three-dimensional nets to capture and kill nematodes. In this study, AoPrdx2, a homologous protein of Prx5, was investigated in via gene disruption, phenotypic analysis, and metabolomics. The deletion of resulted in an increase in the number of mycelial septa and a reduction in the number of nuclei and spore yield. Meanwhile, the absence of increased sensitivity to oxidative stresses, whereas the ∆ mutant strain resulted in higher ROS levels than that of the wild-type (WT) strain. In particular, the inactivation of severely influenced trap formation and pathogenicity; the number of traps produced by the ∆ mutant strain was remarkably reduced and the number of mycelial rings of traps in the ∆ mutant strain was less than that of the WT strain. In addition, the abundance of metabolites in the ∆ mutant strain was significantly downregulated compared with the WT strain. These results indicate that AoPrdx2 plays an indispensable role in the scavenging of ROS, trap morphogenesis, and secondary metabolism.