International journal of molecular sciences, 2021-02, Vol.22 (4), p.1669
2021
Details
- Autor(en) / Beteiligte
- Titel
- The pip1s Quintuple Mutants Demonstrate the Essential Roles of PIP1s in the Plant Growth and Development of Arabidopsis
- Ist Teil von
- International journal of molecular sciences, 2021-02, Vol.22 (4), p.1669
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Plasma membrane intrinsic proteins (PIPs) transport water, CO and small neutral solutes across the plasma membranes. In this study, we used the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 system (CRISPR/Cas9) to mutate and in a triple mutant to generate a ( ) quintuple mutant. Compared to the wild-type (WT) plant, the mutants had smaller sized rosette leaves and flowers, less rosette leaf number, more undeveloped siliques, shorter silique and less seeds. The pollen germination rate of the mutant was significantly lower than that of the WT and the outer wall of the mutant's pollen was deformed. The transcriptomic analysis showed significant alterations in the expression of many key genes and transcription factors (TFs) in the mutant which involved in the development of leaf, flower and pollen, suggesting that the mutant of not only directly affects hydraulics and carbon fixation, but also regulates the expression of related genes to affect plant growth and development.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms22041669Titel-ID: cdi_doaj_primary_oai_doaj_org_article_b3a3a08837114d309820bf319acb161f
- Format
- –
- Schlagworte
- Agricultural production, Aquaporins, Arabidopsis, Arabidopsis - genetics, Arabidopsis - growth & development, Arabidopsis - metabolism, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Carbon dioxide, Carbon fixation, Corn, CRISPR, Efficiency, Flowers, Flowers & plants, Flowers - genetics, Flowers - growth & development, Flowers - metabolism, Fluid dynamics, Fluid flow, Gene expression, Gene Expression Regulation, Plant, Genes, Germination, Hydraulics, Leaves, Localization, Membrane Proteins - genetics, Membrane Proteins - metabolism, Membranes, Mutants, Mutation, Physiology, PIP1s, Plant Development - genetics, Plasma, Plasma membranes, Pollen, Pollen - genetics, Pollen - growth & development, Pollen - metabolism, Protein transport, Proteins, reproductive growth, Rosette, Seeds, Solutes, Transcription factors, transcriptome, vegetative growth