Details

Autor(en) / Beteiligte

• Praktiknjo, Samantha D
• Obermayer, Benedikt
• Zhu, Qionghua
• Fang, Liang
• Liu, Haiyue
• Quinn, Hazel
• Stoeckius, Marlon
• Kocks, Christine
• Birchmeier, Walter
• Rajewsky, Nikolaus

Titel

Tracing tumorigenesis in a solid tumor model at single-cell resolution

Ist Teil von

• Nature communications, 2020-02, Vol.11 (1), p.991-12, Article 991

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek (Open access)

Beschreibungen/Notizen

• Characterizing the complex composition of solid tumors is fundamental for understanding tumor initiation, progression and metastasis. While patient-derived samples provide valuable insight, they are heterogeneous on multiple molecular levels, and often originate from advanced tumor stages. Here, we use single-cell transcriptome and epitope profiling together with pathway and lineage analyses to study tumorigenesis from a developmental perspective in a mouse model of salivary gland squamous cell carcinoma. We provide a comprehensive cell atlas and characterize tumor-specific cells. We find that these cells are connected along a reproducible developmental trajectory: initiated in basal cells exhibiting an epithelial-to-mesenchymal transition signature, tumorigenesis proceeds through Wnt-differential cancer stem cell-like subpopulations before differentiating into luminal-like cells. Our work provides unbiased insights into tumor-specific cellular identities in a whole tissue environment, and emphasizes the power of using defined genetic model systems.