Details

Autor(en) / Beteiligte

• Rakosy-Tican, Elena
• Lörincz-Besenyei, Enikö
• Molnár, Imola
• Thieme, Ramona
• Hartung, Frank
• Sprink, Thorben
• Antonova, Olga
• Famelaer, Ivan
• Angenon, Geert
• Aurori, Adriana

Titel

New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization

Ist Teil von

• Frontiers in plant science, 2019-01, Vol.10, p.3

Ort / Verlag

Switzerland: Frontiers Media S.A

Erscheinungsjahr

2019

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• As plants are sessile they need a very efficient system for repairing damage done by external or internal mutagens to their DNA. Mismatch repair (MMR) is one of the systems that maintain genome integrity and prevent homeologous recombination. In all eukaryotes mismatches are recognized by evolutionary conserved MSH proteins often acting as heterodimers, the constant component of which is MSH2. Changes affecting the function of gene may induce a 'mutator' phenotype and microsatellite instability (MSI), as is demonstrated in knock-out and silenced lines of . The goal of this study was to screen for 'mutator' phenotypes in somatic hybrids between potato cvs. 'Delikat' and 'Désirée' and MMR deficient transformed using antisense (AS) or dominant negative mutant (DN) 2 genes. The results demonstrate that first generation fusion hybrids have a range of morphological abnormalities caused by uniparental MMR deficiency; these mutant phenotypes include: dwarf or gigantic plants; bushiness; curled, small, large or abnormal leaves; a deterioration in chloroplast structure; small deep-purple tubers and early dehiscent flowers. Forty percent of the viable somatic hybrids planted in a greenhouse, (10 out of 25 genotypes) had mutant phenotypes accompanied by MSI. The majority of the hybrids with 'mutator' phenotypes cultured on media containing kanamycin developed roots so sustaining the presence of selectable marker gene II, from the initial constructs. Here for the first time, MMR deficiency combined with somatic hybridization, are used to induce new phenotypes in plants, which supports the role of MMR deficiency in increasing introgressions between two related species.