eLife, 2019-12, Vol.8
2019
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- Autor(en) / Beteiligte
- Titel
- Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1
- Ist Teil von
- eLife, 2019-12, Vol.8
- Ort / Verlag
- England: eLife Sciences Publications Ltd
- Erscheinungsjahr
- 2019
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Cardiac conduction defects decrease life expectancy in myotonic dystrophy type 1 (DM1), a CTG repeat disorder involving misbalance between two RNA binding factors, MBNL1 and CELF1. However, how DM1 condition translates into conduction disorders remains poorly understood. Here we simulated MBNL1 and CELF1 misbalance in the heart and performed TU-tagging-based RNAseq of cardiac cells. We detected deregulations of several genes controlling cellular calcium levels, including increased expression of straightjacket/α2δ3, which encodes a regulatory subunit of a voltage-gated calcium channel. Straightjacket overexpression in the fly heart leads to asynchronous heartbeat, a hallmark of abnormal conduction, whereas cardiac straightjacket knockdown improves these symptoms in DM1 fly models. We also show that ventricular α2δ3 expression is low in healthy mice and humans, but significantly elevated in ventricular muscles from DM1 patients with conduction defects. These findings suggest that reducing ventricular straightjacket/α2δ3 levels could offer a strategy to prevent conduction defects in DM1.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/eLife.51114Titel-ID: cdi_doaj_primary_oai_doaj_org_article_b0487d6d1f624da8b6b94f4c6ec30097
- Format
- –
- Schlagworte
- Animals, Calcium, Calcium channels (voltage-gated), Calcium Channels - biosynthesis, Calcium Channels - genetics, Cardiac arrhythmia, Cardiac Conduction System Disease - genetics, Cardiac Conduction System Disease - physiopathology, Cardiomyocytes, Conduction, conduction defects, Defects, Disease Models, Animal, Drosophila, Drosophila Proteins - biosynthesis, Drosophila Proteins - genetics, Dystrophy, Gene Expression Regulation, Genes, Heart, Human Biology and Medicine, Humans, Insects, Kinases, Life Sciences, Life span, Mice, Muscles, Myotonic dystrophy, Myotonic Dystrophy - complications, myotonic dystrophy type 1, Neurons and Cognition, Proteins, Ribonucleic acid, RNA, TU-tagging, Ventricle