Details

Autor(en) / Beteiligte

• Shan-Shan, Li
• Ip Carman K M
• Tang Matthew Y H
• Tang, Maggie K
• Yin, Tong
• Zhang Jiangwen
• Hassan Ayon Ahmed
• Mak, Abby S
• Yung, Susan
• Tak-Mao, Chan
• Ip, Philip P
• Lee, Cheuk Lun
• Chiu Philip C N
• Lee Leo Tsz On
• Hung-Cheng, Lai
• Jin-Zhang, Zeng
• Shum Ho Cheung
• Wong Alice S T

Titel

Sialyl Lewisx-P-selectin cascade mediates tumor–mesothelial adhesion in ascitic fluid shear flow

Ist Teil von

• Nature communications, 2019-06, Vol.10 (1), p.2406-2406, Article 2406

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Organ-specific colonization suggests that specific cell–cell recognition is essential. Yet, very little is known about this particular interaction. Moreover, tumor cell lodgement requires binding under shear stress, but not static, conditions. Here, we successfully isolate the metastatic populations of cancer stem/tumor-initiating cells (M-CSCs). We show that the M-CSCs tether more and roll slower than the non-metastatic (NM)-CSCs, thus resulting in the preferential binding to the peritoneal mesothelium under ascitic fluid shear stress. Mechanistically, this interaction is mediated by P-selectin expressed by the peritoneal mesothelium. Insulin-like growth factor receptor-1 carrying an uncommon non-sulfated sialyl-Lewisx (sLex) epitope serves as a distinct P-selectin binding determinant. Several glycosyltransferases, particularly α1,3-fucosyltransferase with rate-limiting activity for sLex synthesis, are highly expressed in M-CSCs. Tumor xenografts and clinical samples corroborate the relevance of these findings. These data advance our understanding on the molecular regulation of peritoneal metastasis and support the therapeutic potential of targeting the sLex-P-selectin cascade.Tumor cell in the peritoneum are often exposed to shear forces generated by ascitic flow during metastasis. Here, the authors show that metastatic cancer stem cells tether more and roll slower than the non-metastatic counterparts, and that sialyl-Lewisx -P-selectin axis mediates peritoneal metastasis.