Details

Autor(en) / Beteiligte

• Cheng, Huey Jia
• Ee, Robson
• Cheong, Yuet Meng
• Tan, Wen-Si
• Yin, Wai-Fong
• Chan, Kok-Gan

Titel

Detection of quorum sensing activity in the multidrug-resistant clinical isolate Pseudomonas aeruginosa strain GB11

Ist Teil von

• Sensors (Basel, Switzerland), 2014-07, Vol.14 (7), p.12511-12522

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• A multidrug-resistant clinical bacteria strain GB11 was isolated from a wound swab on the leg of a patient. Identity of stain GB11 as Pseudomonas aeruginosa was validated by using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Detection of the production of signaling molecules, N-acylhomoserine lactones (AHLs), was conducted using three different bacterial biosensors. A total of four different AHLs were found to be produced by strain GB11, namely N-butyryl homoserine lactone (C4-HSL), N-hexanoylhomoserine lactone (C6-HSL), N-octanoyl homoserine lactone (C8-HSL) and N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL) using high resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these detected AHLs, 3-oxo-C12-HSL was found to be the most abundant AHL produced by P. aeruginosa GB11.