BMC genomics, 2024-04, Vol.25 (1), p.376-376
2024
Details
- Autor(en) / Beteiligte
- Titel
- Prominent transcriptomic changes in Mycobacterium intracellulare under acidic and oxidative stress
- Ist Teil von
- BMC genomics, 2024-04, Vol.25 (1), p.376-376
- Ort / Verlag
- England: BioMed Central Ltd
- Erscheinungsjahr
- 2024
- Link zum Volltext
- Quelle
- SpringerLink (Online service)
- Beschreibungen/Notizen
- Mycobacterium avium complex (MAC), including Mycobacterium intracellulare is a member of slow-growing mycobacteria and contributes to a substantial proportion of nontuberculous mycobacterial lung disease in humans affecting immunocompromised and elderly populations. Adaptation of pathogens in hostile environments is crucial in establishing infection and persistence within the host. However, the sophisticated cellular and molecular mechanisms of stress response in M. intracellulare still need to be fully explored. We aimed to elucidate the transcriptional response of M. intracellulare under acidic and oxidative stress conditions. At the transcriptome level, 80 genes were shown [FC] ≥ 2.0 and p < 0.05 under oxidative stress with 10 mM hydrogen peroxide. Specifically, 77 genes were upregulated, while 3 genes were downregulated. In functional analysis, oxidative stress conditions activate DNA replication, nucleotide excision repair, mismatch repair, homologous recombination, and tuberculosis pathways. Additionally, our results demonstrate that DNA replication and repair system genes, such as dnaB, dinG, urvB, uvrD2, and recA, are indispensable for resistance to oxidative stress. On the contrary, 878 genes were shown [FC] ≥ 2.0 and p < 0.05 under acidic stress with pH 4.5. Among these genes, 339 were upregulated, while 539 were downregulated. Functional analysis highlighted nitrogen and sulfur metabolism pathways as the primary responses to acidic stress. Our findings provide evidence of the critical role played by nitrogen and sulfur metabolism genes in the response to acidic stress, including narGHIJ, nirBD, narU, narK3, cysND, cysC, cysH, ferredoxin 1 and 2, and formate dehydrogenase. Our results suggest the activation of several pathways potentially critical for the survival of M. intracellulare under a hostile microenvironment within the host. This study indicates the importance of stress responses in M. intracellulare infection and identifies promising therapeutic targets.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1471-2164eISSN: 1471-2164DOI: 10.1186/s12864-024-10292-4Titel-ID: cdi_doaj_primary_oai_doaj_org_article_ad2a315609c54dd3ad6b8e6a50cc2ccc
- Format
- –
- Schlagworte
- Acidic oxides, Adaptation, Aged, Cellular stress response, Control, Deoxyribonucleic acid, DNA, DNA biosynthesis, DNA repair, DNA replication, Down-regulation, Drug resistance in microorganisms, Ferredoxin, Formate dehydrogenase, Functional analysis, Gene Expression Profiling, Genes, Genomes, Health aspects, Homologous recombination, Humans, Hydrogen peroxide, Identification and classification, Immunocompromised host, Infections, Leprosy, Lung diseases, Metabolism, Microenvironments, Mismatch repair, Molecular modelling, Mycobacteria, Mycobacterium, Mycobacterium avium complex, Mycobacterium avium Complex - genetics, Mycobacterium avium-intracellulare Infection - microbiology, Mycobacterium intracellulare, Nitrogen, Nontuberculous mycobacteria, Nucleotide excision repair, Nucleotides, Oxidation resistance, Oxidative Stress, Pathogens, Patients, Prevention, RecA protein, Recombination, Replication, RNA-seq, Soils, Stress resistance, Sulfur, Sulphur, Therapeutic targets, Transcriptome, Transcriptomes, Transcriptomics, Tuberculosis