Details

Autor(en) / Beteiligte

• Masson, Ana Paula
• Silva, Célio Lopes
• Sartori, Alexandrina
• Marques, Camila
• Balbino, Bianca
• Colavite, Priscila Maria
• da Rosa, Larissa Camargo
• Ishikawa, Larissa Lumi Watanabe
• França, Thais Graziela Donegá
• Chiuso-Minicucci, Fernanda
• Zorzella-Pezavento, Sofia Fernanda Gonçalves
• Ikoma, Maura Rosane Valerio
• Santos, Alessandra

Titel

pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity

Ist Teil von

• Journal of Immunology Research, 2017-01, Vol.2017 (2017), p.1-11

Ort / Verlag

Cairo, Egypt: Hindawi Publishing Corporation

Erscheinungsjahr

2017

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Experimental autoimmune encephalomyelitis (EAE) is a demyelinating pathology of the central nervous system (CNS) used as a model to study multiple sclerosis immunopathology. EAE has also been extensively employed to evaluate potentially therapeutic schemes. Considering the presence of an immune response directed to heat shock proteins (hsps) in autoimmune diseases and the immunoregulatory potential of these molecules, we evaluated the effect of a previous immunization with a genetic vaccine containing the mycobacterial hsp65 gene on EAE development. C57BL/6 mice were immunized with 4 pVAXhsp65 doses and 14 days later were submitted to EAE induction by immunization with myelin oligodendrocyte glycoprotein ( M O G 35 – 55 ) emulsified in Complete Freund’s Adjuvant. Vaccinated mice presented significant lower clinical scores and lost less body weight. M O G 35 – 55 immunization also determined less inflammation in lumbar spinal cord but did not change CD4+CD25+Foxp3+ T cells frequency in spleen and CNS. Infiltrating cells from the CNS stimulated with rhsp65 produced significantly higher levels of IL-10. These results suggest that the ability of pVAXhsp65 vaccination to control EAE development is associated with IL-10 induction.