Details

Autor(en) / Beteiligte

• Ratliff, Miriam
• Kim, Hichul
• Qi, Hao
• Kim, Minsung
• Ku, Bosung
• Azorin, Daniel Dominguez
• Hausmann, David
• Khajuria, Rajiv K.
• Patel, Areeba
• Maier, Elena
• Cousin, Loic
• Ogier, Arnaud
• Sahm, Felix
• Etminan, Nima
• Bunse, Lukas
• Winkler, Frank
• El-Khoury, Victoria
• Platten, Michael
• Kwon, Yong-Jun

Titel

Patient-Derived Tumor Organoids for Guidance of Personalized Drug Therapies in Recurrent Glioblastoma

Ist Teil von

• International journal of molecular sciences, 2022-06, Vol.23 (12), p.6572

Ort / Verlag

Basel: MDPI AG

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek (Open access)

Beschreibungen/Notizen

• An obstacle to effective uniform treatment of glioblastoma, especially at recurrence, is genetic and cellular intertumoral heterogeneity. Hence, personalized strategies are necessary, as are means to stratify potential targeted therapies in a clinically relevant timeframe. Functional profiling of drug candidates against patient-derived glioblastoma organoids (PD-GBO) holds promise as an empirical method to preclinically discover potentially effective treatments of individual tumors. Here, we describe our establishment of a PD-GBO-based functional profiling platform and the results of its application to four patient tumors. We show that our PD-GBO model system preserves key features of individual patient glioblastomas in vivo. As proof of concept, we tested a panel of 41 FDA-approved drugs and were able to identify potential treatment options for three out of four patients; the turnaround from tumor resection to discovery of treatment option was 13, 14, and 15 days, respectively. These results demonstrate that this approach is a complement and, potentially, an alternative to current molecular profiling efforts in the pursuit of effective personalized treatment discovery in a clinically relevant time period. Furthermore, these results warrant the use of PD-GBO platforms for preclinical identification of new drugs against defined morphological glioblastoma features.