SLAS discovery, 2023-10, Vol.28 (7), p.350-354
2023
Details
- Autor(en) / Beteiligte
- Titel
- MSC.sensor: Capturing cancer cell interactions with stroma for functional profiling
- Ist Teil von
- SLAS discovery, 2023-10, Vol.28 (7), p.350-354
- Ort / Verlag
- United States: Elsevier
- Erscheinungsjahr
- 2023
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Mesenchymal stromal cells (MSCs) contribute to the microenvironment regulating normal and malignant hematopoiesis, and thus may support subpopulations of cancer cells to escape therapeutic pressure. Here, we engineered bone marrow MSCs to express a synthetic CD19-sensor receptor to detect and display interacting primary CD19+ leukemia cells in coculture. This implementation provides a versatile platform facilitating ex vivo drug response profiling of primary CD19+ leukemia cells in coculture with high-sensitivity and scalability.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2472-5552eISSN: 2472-5560DOI: 10.1016/j.slasd.2023.08.003Titel-ID: cdi_doaj_primary_oai_doaj_org_article_a9c15e3396154e50a40fc0a5d6404211
- Format
- –
- Schlagworte
- Antigens, CD19 - metabolism, Biosensor, Cell Communication, Cell Line, Tumor, Coculture Techniques, Drug synergy, Ex vivo drug screen, Humans, Leukemia, Leukemia - metabolism, Leukemia - pathology, Mesenchymal Stem Cells - metabolism, Mesenchymal stromal cell, Synthetic Notch receptor, Tumor Microenvironment