Cell reports (Cambridge), 2021-03, Vol.34 (12), p.108902-108902, Article 108902
2021
Details
- Autor(en) / Beteiligte
- Titel
- Transcriptomic characterization of dying hair cells in the avian cochlea
- Ist Teil von
- Cell reports (Cambridge), 2021-03, Vol.34 (12), p.108902-108902, Article 108902
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Sensory hair cells are prone to apoptosis caused by various drugs including aminoglycoside antibiotics. In mammals, this vulnerability results in permanent hearing loss because lost hair cells are not regenerated. Conversely, hair cells regenerate in birds, making the avian inner ear an exquisite model for studying ototoxicity and regeneration. Here, we use single-cell RNA sequencing and trajectory analysis on control and dying hair cells after aminoglycoside treatment. Interestingly, the two major subtypes of avian cochlear hair cells, tall and short hair cells, respond differently. Dying short hair cells show a noticeable transient upregulation of many more genes than tall hair cells. The most prominent gene group identified is associated with potassium ion conductances, suggesting distinct physiological differences. Moreover, the dynamic characterization of >15,000 genes expressed in tall and short avian hair cells during their apoptotic demise comprises a resource for further investigations toward mammalian hair cell protection and hair cell regeneration. [Display omitted] •In vivo model to study cochlear hair cell death•Cochlear hair cells die rapidly and synchronously•Single-cell RNA-sequencing reveals two stages of hair cell demise•Major types of dying cochlear hair cells express different sets of genes Benkafadar et al. show a temporal trajectory of gene expression changes that happen when cochlear sensory hair cells are exposed to sisomicin. The in situ-validated data show that apoptosis of the major hair cell subtypes happens via distinctively coordinated programs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2211-1247eISSN: 2211-1247DOI: 10.1016/j.celrep.2021.108902Titel-ID: cdi_doaj_primary_oai_doaj_org_article_a85a3740876c4ac8ab890a919e70bc2b
- Format
- –
- Schlagworte
- aminoglycoside, Aminoglycosides - pharmacology, Animals, apoptosis, Auditory periphery, Cell Death - drug effects, Cell Death - genetics, Chickens - genetics, Gene Expression Profiling, Gene Expression Regulation - drug effects, Hair Cells, Auditory - drug effects, Hair Cells, Auditory - pathology, hearing, inner ear, ototoxicity, scRNA-seq, Semicircular Canals - drug effects, Semicircular Canals - metabolism, Sisomicin - administration & dosage, Sisomicin - pharmacology, Time Factors, Transcriptome - drug effects, Transcriptome - genetics