Biomedicines, 2021-07, Vol.9 (8), p.868
2021
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- Autor(en) / Beteiligte
- Titel
- Immune Assessment of BNT162b2 m-RNA-Spike Based Vaccine Response in Adults
- Ist Teil von
- Biomedicines, 2021-07, Vol.9 (8), p.868
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2021
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Vaccine efficacy is based on clinical data. Currently, the assessment of immune response after SARS-CoV-2 vaccination is scarce. A total of 52 healthcare workers were immunized with the same lot of BNT162b2 vaccine. The immunological response against the vaccine was tested using a T-specific assay based on the expression of CD25 and CD134 after stimulation with anti-N, -S, and -M specific peptides of SARS-CoV-2. Moreover, IgG anti-S2 and -RBD antibodies were detected using ELISA. Furthermore, the cell subsets involved in the response to the vaccine were measured in peripheral blood by flow cytometry. Humoral-specific responses against the vaccine were detected in 94% and 100% after the first and second doses, respectively. Therefore, anti-S T-specific responses were observed in 57% and 90% of the subjects after the first and second doses of the vaccine, respectively. Thirty days after the second dose, significant increases in T helper 1 memory cells ( < 0.001), peripheral memory T follicular helper (pT ) cells ( < 0.032), and switched memory ( = 0.005) were observed. This study describes the specific humoral and cellular immune responses after vaccination with the new mRNA-based BNT162b2 vaccine. A mobilization of T into the circulation occurs, reflecting a specific activation of the immune system.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2227-9059eISSN: 2227-9059DOI: 10.3390/biomedicines9080868Titel-ID: cdi_doaj_primary_oai_doaj_org_article_a74cfb5dbe7e4a70a91d544175a3cf4b
- Format
- –
- Schlagworte
- Antigens, B-specific response, CD134 antigen, CD25 antigen, Clinical trials, Cloning, Coronaviruses, COVID-19 vaccines, Enzyme-linked immunosorbent assay, Flow cytometry, Immune response (cell-mediated), Immune response (humoral), Immunoglobulin G, Infections, Lymphocytes, Medical personnel, Memory cells, Monoclonal antibodies, mRNA, Peripheral blood, Proteins, SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2, Statistical analysis, T follicular helper cells, T-specific response, Vaccination, Vaccine efficacy, Vaccines