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Enterococcus faecalis AHG0090 is a Genetically Tractable Bacterium and Produces a Secreted Peptidic Bioactive that Suppresses Nuclear Factor Kappa B Activation in Human Gut Epithelial Cells
Ist Teil von
Frontiers in immunology, 2018-04, Vol.9, p.790
Ort / Verlag
Switzerland: Frontiers Media S.A
Erscheinungsjahr
2018
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
is an early coloniser of the human infant gut and contributes to the development of intestinal immunity. To better understand the functional capacity of
, we constructed a broad host range RP4 mobilizable vector, pEHR513112, that confers chloramphenicol resistance and used a metaparental mating approach to isolate
AHG0090 from a fecal sample collected from a healthy human infant. We demonstrated that
AHG0090 is genetically tractable and could be manipulated using traditional molecular microbiology approaches.
AHG0090 was comparable to the gold-standard anti-inflammatory bacterium
A2-165 in its ability to suppress cytokine-mediated nuclear factor kappa B (NF-κB) activation in human gut-derived LS174T goblet cell like and Caco-2 enterocyte-like cell lines.
AHG0090 and
A2-165 produced secreted low molecular weight NF-κB suppressive peptidic bioactives. Both bioactives were sensitive to heat and proteinase K treatments although the
AHG0090 bioactive was more resilient to both forms of treatment. As expected,
AHG0090 suppressed IL-1β-induced NF-κB-p65 subunit nuclear translocation and expression of the NF-κB regulated genes IL-6, IL-8 and CXCL-10. Finally, we determined that
AHG0090 is distantly related to other commensal strains and likely encodes niche factors that support effective colonization of the infant gut.