Details

Autor(en) / Beteiligte

• Raj, Kaushik
• Partow, Siavash
• Correia, Kevin
• Khusnutdinova, Anna N.
• Yakunin, Alexander F.
• Mahadevan, Radhakrishnan

Titel

Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae

Ist Teil von

• Metabolic engineering communications, 2018-06, Vol.6, p.28-32

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Adipic acid is an important industrial chemical used in the synthesis of nylon-6,6. The commercial synthesis of adipic acid uses petroleum-derived benzene and releases significant quantities of greenhouse gases. Biocatalytic production of adipic acid from renewable feedstocks could potentially reduce the environmental damage and eliminate the need for fossil fuel precursors. Recently, we have demonstrated the first enzymatic hydrogenation of muconic acid to adipic acid using microbial enoate reductases (ERs) - complex iron-sulfur and flavin containing enzymes. In this work, we successfully expressed the Bacillus coagulans ER in a Saccharomyces cerevisiae strain producing muconic acid and developed a three-stage fermentation process enabling the synthesis of adipic acid from glucose. The ability to express active ERs and significant acid tolerance of S. cerevisiae highlight the applicability of the developed yeast strain for the biocatalytic production of adipic acid from renewable feedstocks. •An enzyme capable of reducing α pi bonds in carboxylic acids has been expressed in S. cerevisiae.•The first yeast strain capable of complete adipic acid biosynthesis has been developed.•A three-stage fermentation strategy has been proposed to convert glucose to adipic acid.