Details

Autor(en) / Beteiligte

• Qadir, Mehmood
• Faryal, Rani
• Khan, Muhammad Tahir
• Khan, Sajjad Ahmed
• Zhang, Shulin
• Li, Weimin
• Wei, Dong Qing
• Tahseen, Sabira
• McHugh, Timothy D
• Craney, Arryn

Titel

Phenotype versus genotype discordant rifampicin susceptibility testing in tuberculosis: implications for a diagnostic accuracy

Ist Teil von

• Microbiology spectrum, 2024-01, Vol.12 (1), p.e0163123-e0163123

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• An accurate diagnosis of drug resistance in clinical isolates is an important step for better treatment outcomes. The current study observed a higher discordance rate of rifampicin resistance on Mycobacteria Growth Indicator Tube (MGIT) drug susceptibility testing (DST) than Lowenstein-Jenson (LJ) DST when compared with the sequencing. We detected a few novel mutations and their combination in rifampicin resistance isolates that were missed by MGIT DST and may be useful for the better management of tuberculosis (TB) treatment outcomes. Few novel deletions in clinical isolates necessitate the importance of sequencing in large data sets in geographic-specific locations, especially high-burden countries. We explored the discordance rate on MGIT and LJ, which is important for the clinical management of rifampicin resistance to avoid the mistreatment of drug-resistant TB. Furthermore, MGIT-sensitive isolates may be subjected to molecular methods of diagnosis for further confirmation and treatment options.