Molecules (Basel, Switzerland), 2021-01, Vol.26 (2), p.289
2021
Details
- Autor(en) / Beteiligte
- Titel
- The Impact of Olive Oil Compounds on the Metabolic Reprogramming of Cutaneous Melanoma Cell Models
- Ist Teil von
- Molecules (Basel, Switzerland), 2021-01, Vol.26 (2), p.289
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cutaneous melanoma is the deadliest type of skin cancer, characterized by a high molecular and metabolic heterogeneity which contributes to therapy resistance. Despite advances in treatment, more efficient therapies are needed. Olive oil compounds have been described as having anti-cancer properties. Here, we clarified the cytotoxic potential of oleic acid, homovanillyl alcohol, and hydroxytyrosol on melanoma cells. Metabolic viability was determined 48 h post treatment of A375 and MNT1 cells. Metabolic gene expression was assessed by qRT-PCR and Mitogen-Activated Protein Kinase (MAPK) activation by Western blot. Hydroxytyrosol treatment (100 and 200 µM) significantly reduced A375 cell viability ( = 0.0249; < 0.0001) which, based on the expression analysis performed, is more compatible with a predominant glycolytic profile and c-Jun N-terminal kinase (JNK) activation. By contrast, hydroxytyrosol had no effect on MNT1 cell viability, which demonstrates an enhanced oxidative metabolism and extracellular signal-regulated kinase (ERK) activation. This compound triggered cell detoxification and the use of alternative energy sources in A375 cells, inhibiting JNK and ERK pathways. Despite oleic acid and homovanillyl alcohol demonstrating no effect on melanoma cell viability, they influenced the MNT1 glycolytic rate and A375 detoxification mechanisms, respectively. Both compounds suppressed ERK activation in MNT1 cells. The distinct cell responses to olive oil compounds depend on the metabolic and molecular mechanisms preferentially activated. Hydroxytyrosol may have a cytotoxic potential in melanoma cells with predominant glycolytic metabolism and JNK activation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1420-3049eISSN: 1420-3049DOI: 10.3390/molecules26020289Titel-ID: cdi_doaj_primary_oai_doaj_org_article_a3aa4bd06e0948759fa7a227a7b6542a
- Format
- –
- Schlagworte
- Alcohol, Alternative energy, Alternative energy sources, Antineoplastic Agents, Phytogenic - chemistry, Antineoplastic Agents, Phytogenic - pharmacology, c-Jun protein, Cancer therapies, Cell activation, Cell culture, Cell Line, Tumor, cell metabolism, Cell Survival - drug effects, Cell viability, Cellular Reprogramming Techniques, cutaneous melanoma, Cytotoxicity, Dehydrogenases, Detoxification, Energy sources, Extracellular signal-regulated kinase, Gene expression, Glucose, Glycolysis, Heterogeneity, homovanillyl alcohol, Humans, hydroxytyrosol, Immunotherapy, JNK protein, Kinases, MAP kinase, MAP Kinase Signaling System - drug effects, Melanoma, Melanoma - drug therapy, Melanoma - metabolism, Melanoma - pathology, Metabolic pathways, Metabolism, Metabolites, molecular mechanisms, Molecular modelling, Neoplasm Proteins - metabolism, Older people, Oleic acid, Olive oil, Olive Oil - chemistry, Oxidative metabolism, Oxidative stress, Phenylethyl Alcohol - analogs & derivatives, Phenylethyl Alcohol - chemistry, Phenylethyl Alcohol - pharmacology, Protein kinase, Proteins, Sarcoma, Skin cancer, Skin Neoplasms - drug therapy, Skin Neoplasms - metabolism, Skin Neoplasms - pathology, Transcription factors