BMC genomics, 2024-05, Vol.25 (1), p.543-11, Article 543
2024
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- Autor(en) / Beteiligte
- Titel
- MIR396-GRF/GIF enhances in planta shoot regeneration of Dendrobium catenatum
- Ist Teil von
- BMC genomics, 2024-05, Vol.25 (1), p.543-11, Article 543
- Ort / Verlag
- England: BioMed Central Ltd
- Erscheinungsjahr
- 2024
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Recent studies on co-transformation of the growth regulator, TaGRF4-GIF1 chimera (Growth Regulating Factor 4-GRF Interacting Factor 1), in cultivated wheat varieties (Triticum aestivum), showed improved regeneration efficiency, marking a significant breakthrough. Here, a simple and reproducible protocol using the GRF4-GIF1 chimera was established and tested in the medicinal orchid Dendrobium catenatum, a monocot orchid species. TaGRF4-GIF1 from T. aestivum and DcGRF4-GIF1 from D. catenatum were reconstructed, with the chimeras significantly enhancing the regeneration efficiency of D. catenatum through in planta transformation. Further, mutating the microRNA396 (miR396) target sites in TaGRF4 and DcGRF4 improved regeneration efficiency. The target mimicry version of miR396 (MIM396) not only boosted shoot regeneration but also enhanced plant growth. Our methods revealed a powerful tool for the enhanced regeneration and genetic transformation of D. catenatum.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1471-2164eISSN: 1471-2164DOI: 10.1186/s12864-024-10360-9Titel-ID: cdi_doaj_primary_oai_doaj_org_article_a296d59ab6da4c31b2a6edb827a38b96
- Format
- –
- Schlagworte
- Analysis, Chimeras, Cultivation, Dendrobium - genetics, Dendrobium - growth & development, Dendrobium catenatum, Diseases and pests, Efficiency, Flowers & plants, Gene expression, Genes, Genetic aspects, Genetic transformation, Genetically altered foods, Genomes, Genotype & phenotype, Grain cultivation, Growth, Growth regulators, Health aspects, Measurement, Medicinal plants, MicroRNAs - genetics, MicroRNAs - metabolism, Mimicry, miRNA, Monocotyledonous species, Mutation, Orchid, Orchids, Phylogenetics, Plant growth, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Shoots - genetics, Plant Shoots - growth & development, Plants, Genetically Modified - genetics, Regeneration, Regeneration - genetics, Somatotropin releasing hormone, Transcription factors, Transformation, Transgenic plants, Triticum aestivum, Wheat