Details

Autor(en) / Beteiligte

• Doi, Daisuke
• Magotani, Hiroaki
• Kikuchi, Tetsuhiro
• Ikeda, Megumi
• Hiramatsu, Satoe
• Yoshida, Kenji
• Amano, Naoki
• Nomura, Masaki
• Umekage, Masafumi
• Morizane, Asuka
• Takahashi, Jun

Titel

Pre-clinical study of induced pluripotent stem cell-derived dopaminergic progenitor cells for Parkinson's disease

Ist Teil von

• Nature communications, 2020-07, Vol.11 (1), p.3369-3369, Article 3369

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Induced pluripotent stem cell (iPSC)-derived dopaminergic (DA) neurons are an expected source for cell-based therapies for Parkinson's disease (PD). The regulatory criteria for the clinical application of these therapies, however, have not been established. Here we show the results of our pre-clinical study, in which we evaluate the safety and efficacy of dopaminergic progenitors (DAPs) derived from a clinical-grade human iPSC line. We confirm the characteristics of DAPs by in vitro analyses. We also verify that the DAP population include no residual undifferentiated iPSCs or early neural stem cells and have no genetic aberration in cancer-related genes. Furthermore, in vivo studies using immunodeficient mice reveal no tumorigenicity or toxicity of the cells. When the DAPs are transplanted into the striatum of 6-OHDA-lesioned rats, the animals show behavioral improvement. Based on these results, we started a clinical trial to treat PD patients in 2018.