International journal of molecular sciences, 2023-02, Vol.24 (4), p.4162
2023
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- Autor(en) / Beteiligte
- Titel
- The Colorimetric Detection of the Hydroxyl Radical
- Ist Teil von
- International journal of molecular sciences, 2023-02, Vol.24 (4), p.4162
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2023
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- An aromatic substrate for hydroxylation by hydroxyl radicals ( OH) was investigated. The probe, '-(5-nitro-1,3-phenylene)- -glutaramide, and its hydroxylated product do not bind either iron(III) or iron(II), and so they do not interfere with the Fenton reaction. A spectrophotometric assay based on the hydroxylation of the substrate was developed. The synthesis and purification methods of this probe from previously published methodologies were improved upon, as well as the analytical procedure for monitoring the Fenton reaction through its use, enabling univocal and sensitive OH detection. The assay was utilised to demonstrate that the iron(III) complexes of long-chain fatty acids lack Fenton activity under biological conditions.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms24044162Titel-ID: cdi_doaj_primary_oai_doaj_org_article_a0ebe45a363e44c280da6f017f0c4b37
- Format
- –
- Schlagworte
- Amino acids, aromatic hydroxylation, Biological activity, Colorimetry, Communication, Electron transport, Ethylenediaminetetraacetic acid, Fatty acids, Fenton activity, Fenton reaction, Ferric Compounds, Free radicals, Hydrogen Peroxide - chemistry, Hydroxides, hydroxyl radical, Hydroxyl Radical - chemistry, Hydroxyl radicals, Hydroxylation, Iron, Iron - chemistry, Iron Chelating Agents, iron complexes, Ligands, Oxidation, Spectrophotometry, Substrates