Details

Autor(en) / Beteiligte

• Yuan, Xinyue
• Schröter, Manuel
• Obien, Marie Engelene J
• Fiscella, Michele
• Gong, Wei
• Kikuchi, Tetsuhiro
• Odawara, Aoi
• Noji, Shuhei
• Suzuki, Ikuro
• Takahashi, Jun
• Hierlemann, Andreas
• Frey, Urs

Titel

Versatile live-cell activity analysis platform for characterization of neuronal dynamics at single-cell and network level

Ist Teil von

• Nature communications, 2020-09, Vol.11 (1), p.4854-4854, Article 4854

Ort / Verlag

England: Nature Publishing Group UK

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Chronic imaging of neuronal networks in vitro has provided fundamental insights into mechanisms underlying neuronal function. Current labeling and optical imaging methods, however, cannot be used for continuous and long-term recordings of the dynamics and evolution of neuronal networks, as fluorescent indicators can cause phototoxicity. Here, we introduce a versatile platform for label-free, comprehensive and detailed electrophysiological live-cell imaging of various neurogenic cells and tissues over extended time scales. We report on a dual-mode high-density microelectrode array, which can simultaneously record in (i) full-frame mode with 19,584 recording sites and (ii) high-signal-to-noise mode with 246 channels. We set out to demonstrate the capabilities of this platform with recordings from primary and iPSC-derived neuronal cultures and tissue preparations over several weeks, providing detailed morpho-electrical phenotypic parameters at subcellular, cellular and network level. Moreover, we develop reliable analysis tools, which drastically increase the throughput to infer axonal morphology and conduction speed.