Details

Autor(en) / Beteiligte

• Chen, Xu
• Zhao, Meijiao
• Xie, Qihu
• Zhou, Sitong
• Zhong, Xiaoping
• Zheng, Judun
• Yang, Ronghua
• Du, Xianjin
• Xia, Jinyu
• Liao, Yuhui

Titel

Click‐hydrogel delivered aggregation‐induced emissive nanovesicles for simultaneous remodeling and antibiosis of deep burn wounds

Ist Teil von

• Aggregate (Hoboken), 2024-02, Vol.5 (1), p.n/a

Ort / Verlag

Guangzhou: John Wiley & Sons, Inc

Erscheinungsjahr

2024

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• As a high‐risk trauma, deep burns are always hindered in their repair process by decreased tissue regeneration capacity and persistent infections. In this study, we developed a simultaneous strategy for deep burn wounds treatment using functional nanovesicles with antibacterial and tissue remodeling properties, delivered via a click‐chemistry hydrogel. An aggregation‐induced emission photosensitizer of 4‐(2‐(5‐(4‐(diphenylamino)phenyl)thiophen‐2‐yl)vinyl)‐1‐(2‐hydroxyethyl) pyridin‐1‐ium bromide (THB) with excellent photodynamic properties was first prepared, and then combined with readily accessible adipose stem cells‐derived nanovesicles to generate the THB functionalized nanovesicles (THB@ANVs). The THB@ANVs showed strong antibacterial activity against Gram‐positive bacteria (up to 100% killing rate), and also beneficial effects on tissue remodeling, including promoting cell migration, cell proliferation, and regulating immunity. In addition, we prepared a click‐hydrogel of carboxymethyl chitosan for effective delivery of THB@ANVs on wounds. This hydrogel could be injected to conform to the wound morphology while responding to the acidic microenvironment. In vivo evaluations of wound healing revealed that the THB@ANVs hydrogel dressing efficiently accelerated the healing of second‐degree burn wounds by reducing bacterial growth, regulating inflammation, promoting early angiogenesis, and collagen deposition. This study provides a promising candidate of wound dressing with diverse functions for deep burn wound repair. To achieve simultaneous photodynamic antibacterial activity and tissue remodeling effects for deep burn wound healing, adult adipose‐derived stem cells‐derived nanovesicles were combined with 4‐(2‐(5‐(4‐(diphenylamino)phenyl)thiophen‐2‐yl)vinyl)‐1‐(2‐hydroxyethyl) pyridin‐1‐ium bromide (THB) of aggregation‐induced emission property to form THB@ANVs. Injectable click‐chemistry hydrogel of carboxymethyl chitosan were used to responsively release THB@ANVs on burn wounds, showing significant antibacterial, immunity regulating, and neovascularization promoting effects.