Nature communications, 2021-05, Vol.12 (1), p.3082-3082, Article 3082
2021
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- Autor(en) / Beteiligte
- Titel
- Activation of Prp28 ATPase by phosphorylated Npl3 at a critical step of spliceosome remodeling
- Ist Teil von
- Nature communications, 2021-05, Vol.12 (1), p.3082-3082, Article 3082
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Splicing, a key step in the eukaryotic gene-expression pathway, converts precursor messenger RNA (pre-mRNA) into mRNA by excising introns and ligating exons. This task is accomplished by the spliceosome, a macromolecular machine that must undergo sequential conformational changes to establish its active site. Each of these major changes requires a dedicated DExD/H-box ATPase, but how these enzymes are activated remain obscure. Here we show that Prp28, a yeast DEAD-box ATPase, transiently interacts with the conserved 5' splice-site (5'SS) GU dinucleotide and makes splicing-dependent contacts with the U1 snRNP protein U1C, and U4/U6.U5 tri-snRNP proteins, Prp8, Brr2, and Snu114. We further show that Prp28's ATPase activity is potentiated by the phosphorylated Npl3, but not the unphosphorylated Npl3, thus suggesting a strategy for regulating DExD/H-box ATPases. We propose that Npl3 is a functional counterpart of the metazoan-specific Prp28 N-terminal region, which can be phosphorylated and serves as an anchor to human spliceosome.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-021-23459-4Titel-ID: cdi_doaj_primary_oai_doaj_org_article_9e81c7d52d55433ab0c6e27d3c001065
- Format
- –
- Schlagworte
- Adenosine triphosphatase, Adenosine Triphosphate - metabolism, Amino acids, Antibodies, DEAD-box RNA Helicases - genetics, DEAD-box RNA Helicases - metabolism, DNA helicase, Enzymes, Exons, Experiments, Gene expression, Humans, Hypotheses, Introns, Macromolecules, Multiprotein Complexes - genetics, Multiprotein Complexes - metabolism, Mutation, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Phosphorylation, Protein Binding, Proteins, Ribonuclease H - metabolism, Ribonucleoproteins (small nuclear), Ribonucleoproteins, Small Nuclear - metabolism, RNA Helicases - genetics, RNA Helicases - metabolism, RNA Precursors - genetics, RNA Precursors - metabolism, RNA Splicing, RNA-Binding Proteins - genetics, RNA-Binding Proteins - metabolism, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins - genetics, Saccharomyces cerevisiae Proteins - metabolism, Spliceosomes - genetics, Spliceosomes - metabolism, Splicing, Transfer RNA, Yeast, Yeasts