Details

Autor(en) / Beteiligte

• Isabela da Costa César
• Fernão Castro Braga
• Cristina Duarte Vianna-Soares
• Elzíria de Aguiar Nunan
• Gérson Antônio Pianetti
• Ligia Maria Moreira-Campos

Titel

title language="eng">Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method

Ist Teil von

• Química nova, 2008-01, Vol.31 (8), p.1933-1936

Ort / Verlag

Sociedade Brasileira de Química

Erscheinungsjahr

2008

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• This paper describes the development and validation of an UV-Visible spectrophotometric method for quantitation of genistein and genistin in soy dry extracts, after reaction with aluminum chloride. The method showed to be linear (r²= 0.9999), precise (R.S.D. < 2%), accurate (recovery of 101.56%) and robust. Seven samples of soy dry extracts were analyzed by the spectrophotometric validated method and by RP-HPLC. Genistein concentrations determined by spectrophotometry (0.63% - 16.05%) were slightly higher than values obtained by HPLC analysis (0.40% - 12.79%); however, the results of both methods showed a strong correlation.