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ZmACY-1 Antagonistically Regulates Growth and Stress Responses in Nicotiana benthamiana
Ist Teil von
Frontiers in plant science, 2021-07, Vol.12, p.593001-593001
Ort / Verlag
Frontiers Media S.A
Erscheinungsjahr
2021
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
Aminoacylase-1 is a zinc-binding enzyme that is important in urea cycling, ammonia scavenging, and oxidative stress responses in animals. Aminoacylase-1 (
ACY-1
) has been reported to play a role in resistance to pathogen infection in the model plant
Nicotiana benthamiana
. However, little is known about its function in plant growth and abiotic stress responses. In this study, we cloned and analyzed expression patterns of
ZmACY-1
in
Zea mays
under different conditions. We also functionally characterized
ZmACY-1
in
N. benthamiana
. We found that
ZmACY-1
is expressed specifically in mature shoots compared with other tissues.
ZmACY-1
is repressed by salt, drought, jasmonic acid, and salicylic acid, but is induced by abscisic acid and ethylene, indicating a potential role in stress responses and plant growth. The overexpression of
ZmACY-1
in
N. benthamiana
promoted growth rate by promoting growth-related genes, such as
NbEXPA1
and
NbEIN2
. At the same time, the overexpression of
ZmACY-1
in
N. benthamiana
reduced tolerance to drought and salt stress. With drought and salt stress, the activity of protective enzymes, such as peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) from micrococcus lysodeikticus was lower; while the content of malondialdehyde (MDA) and relative electrolytic leakage was higher in
ZmACY-1
overexpression lines than that in wild-type lines. The results indicate that
ZmACY-1
plays an important role in the balance of plant growth and defense and can be used to assist plant breeding under abiotic stress conditions.