Details

Autor(en) / Beteiligte

• Zhao, Yan-Jun
• Lv, Hu
• Xu, Ping-Bo
• Zhu, Min-Min
• Liu, Yi
• Miao, Chang-Hong
• Zhu, Yun

Titel

Protective effects of oridonin on the sepsis in mice

Ist Teil von

• The Kaohsiung journal of medical sciences, 2016-09, Vol.32 (9), p.452-457

Ort / Verlag

China (Republic : 1949- ): Elsevier Taiwan

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Abstract This study aimed to investigate the protective effects of oridonin (ORI) on cecal ligation and puncture (CLP)-induced sepsis in mice. Male C57BL/6 mice weighing 22–30 g and aged 8–10 weeks were randomly assigned to three groups: Sham group, CLP group, or CLP plus ORI group. In the CLP group and ORI group, CLP was induced, and intraperitoneal injection of normal saline and oridonin (100 μg/kg) was conducted, respectively. The survival rate was determined within the following 7 days. The blood, liver, and lung were collected at 24 hours after injury. Hematoxylin–eosin staining of the lung, detection of lung wet-to-dry ratio, and serum cytokines (tumor necrosis factor [TNF]-α and interleukin [IL]-6), and examination of intraperitoneal and blood bacterial clearance were conducted to evaluate the therapeutic efficacy. Results showed that ORI treatment significantly reduced the lung wet-to-dry ratio, decreased serum TNF-α and IL-6, and improved liver pathology compared with the CLP group ( p  < 0.05). Moreover, the intraperitoneal and blood bacterial clearance increased markedly after ORI treatment ( p  < 0.05). The 7-day survival rate in the ORI group was also dramatically higher than in the CLP group ( p  < 0.05). Our findings indicate that ORI can attenuate liver and lung injuries and elevate bacterial clearance to increase the survival rate of sepsis mice.