Nature communications, 2021-03, Vol.12 (1), p.1411-1411, Article 1411
2021
Details
- Autor(en) / Beteiligte
- Titel
- Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli
- Ist Teil von
- Nature communications, 2021-03, Vol.12 (1), p.1411-1411, Article 1411
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Genetically programmed circuits allowing bifunctional dynamic regulation of enzyme expression have far-reaching significances for various bio-manufactural purposes. However, building a bio-switch with a post log-phase response and reversibility during scale-up bioprocesses is still a challenge in metabolic engineering due to the lack of robustness. Here, we report a robust thermosensitive bio-switch that enables stringent bidirectional control of gene expression over time and levels in living cells. Based on the bio-switch, we obtain tree ring-like colonies with spatially distributed patterns and transformer cells shifting among spherical-, rod- and fiber-shapes of the engineered Escherichia coli. Moreover, fed-batch fermentations of recombinant E. coli are conducted to obtain ordered assembly of tailor-made biopolymers polyhydroxyalkanoates including diblock- and random-copolymer, composed of 3-hydroxybutyrate and 4-hydroxybutyrate with controllable monomer molar fraction. This study demonstrates the possibility of well-organized, chemosynthesis-like block polymerization on a molecular scale by reprogrammed microbes, exemplifying the versatility of thermo-response control for various practical uses.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-021-21654-xTitel-ID: cdi_doaj_primary_oai_doaj_org_article_9a7310b9724846f194fd87d843879935
- Format
- –
- Schlagworte
- 3-Hydroxybutyric Acid - metabolism, Batch culture, Biopolymers, Cell size, Chemosynthesis, Circuits, Colonies, Copolymers, Dynamic control, E coli, Escherichia coli, Escherichia coli - genetics, Escherichia coli - growth & development, Escherichia coli - metabolism, Fermentation, Gene expression, Gene Expression Regulation, Bacterial, Green Fluorescent Proteins - genetics, Green Fluorescent Proteins - metabolism, Hydroxybutyrates - metabolism, Luminescent Proteins - genetics, Luminescent Proteins - metabolism, Metabolic engineering, Metabolic Engineering - methods, Microorganisms, Genetically-Modified, Polyesters - metabolism, Polyhydroxyalkanoates, Polyhydroxyalkanoates - metabolism, Polymers, Red Fluorescent Protein, Stability, Temperature, Time-Lapse Imaging, Tree rings, γ-Hydroxybutyric acid