Details

Autor(en) / Beteiligte

• Dudka, Damian
• Noatynska, Anna
• Smith, Chris A.
• Liaudet, Nicolas
• McAinsh, Andrew D.
• Meraldi, Patrick

Titel

Complete microtubule–kinetochore occupancy favours the segregation of merotelic attachments

Ist Teil von

• Nature communications, 2018-05, Vol.9 (1), p.2042-16, Article 2042

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Kinetochores are multi-protein complexes that power chromosome movements by tracking microtubules plus-ends in the mitotic spindle. Human kinetochores bind up to 20 microtubules, even though single microtubules can generate sufficient force to move chromosomes. Here, we show that high microtubule occupancy at kinetochores ensures robust chromosome segregation by providing a strong mechanical force that favours segregation of merotelic attachments during anaphase. Using low doses of the microtubules-targeting agent BAL27862 we reduce microtubule occupancy and observe that spindle morphology is unaffected and bi-oriented kinetochores can still oscillate with normal intra-kinetochore distances. Inter-kinetochore stretching is, however, dramatically reduced. The reduction in microtubule occupancy and inter-kinetochore stretching does not delay satisfaction of the spindle assembly checkpoint or induce microtubule detachment via Aurora-B kinase, which was so far thought to release microtubules from kinetochores under low stretching. Rather, partial microtubule occupancy slows down anaphase A and increases incidences of lagging chromosomes due to merotelically attached kinetochores. Single microtubules (MTs) can move chromosomes, but it is unclear why kinetochores bind up to 20 MTs. Here, the authors decrease the number of kinetochore MTs with BAL27862 and see lagging chromosomes, suggesting that numerous kinetochore MTs provide force ensuring robust chromosomal segregation.