Molecules (Basel, Switzerland), 2021-11, Vol.26 (22), p.7014
2021
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- Autor(en) / Beteiligte
- Titel
- Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes
- Ist Teil von
- Molecules (Basel, Switzerland), 2021-11, Vol.26 (22), p.7014
- Ort / Verlag
- Basel: MDPI AG
- Erscheinungsjahr
- 2021
- Quelle
- EZB Free E-Journals
- Beschreibungen/Notizen
- Psoriasis is a chronic inflammatory skin condition characterized by abnormal keratinocyte proliferation and differentiation that is accompanied with dysregulated immune response and abnormal vascularization. Devil’s claw (Harpagophytum procumbens (Burch.) DC. ex Meisn.) tubers extract has been used both systemically and topically for treatment of chronic inflammatory diseases such as arthritis, osteoporosis, inflammatory bowel disease, among others. However, its potential mechanisms of action against psoriasis remains poorly investigated. The human keratinocyte HaCaT cell line is a well-accepted in vitro model system for inflammatory skin disorders such as psoriasis. The present study involved an exploration of the effect of biotechnologically produced H. procumbens (HP) cell suspension extract and pure phenylethanoid glycosides verbascoside (VER) and leucosceptoside A (LEU) in interferon (IFN)-γ/interleukin (IL)-17A/IL-22-stimulated HaCaT cells as a model of psoriasis-like inflammation. Changes in key inflammatory signaling pathways related to psoriasis development were detected by reverse transcription polymerase chain reaction and western blotting. Treatment with LEU, but not VER and HP extract improved psoriasis-related inflammation via suppression of the PI3K/AKT signaling in IFN-γ/IL-17A/IL-22-stimulated HaCaT cells. Our results suggest that LEU may exhibit therapeutic potential against psoriasis by regulating keratinocyte differentiation through inhibition of the PI3K/AKT pathway.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1420-3049eISSN: 1420-3049DOI: 10.3390/molecules26227014Titel-ID: cdi_doaj_primary_oai_doaj_org_article_99b65710ec314300a165d3fbc4a59782
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, AKT protein, Arthritis, Biomedical materials, Biotechnology, devil’s claw, Differentiation, Drug delivery systems, Drugs, Gene expression, Glycosides, Health services, Immune response, Inflammation, Inflammatory bowel diseases, Inflammatory diseases, Interferon, Interleukin 22, Keratin, Keratinocytes, Kinases, leucosceptoside A, Ligands, Metabolites, Natural products, Osteoporosis, Pathophysiology, Phytochemicals, Polymerase chain reaction, Proteins, Psoriasis, Reverse transcription, Signal transduction, Skin, Skin diseases, Steroids, Tubers, Tumor necrosis factor-TNF, Vascularization, verbascoside, Western blotting, γ-Interferon