Nature communications, 2020-04, Vol.11 (1), p.1711-1711, Article 1711
2020
Details
- Autor(en) / Beteiligte
- Titel
- Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages
- Ist Teil von
- Nature communications, 2020-04, Vol.11 (1), p.1711-1711, Article 1711
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation of the ability of organoid culture technology to preserve complex stem/progenitor and differentiated cell types via long-term propagation of normal human mammary tissues. Basal/stem and luminal progenitor cells can differentiate in culture to generate mature basal and luminal cell types, including ER+ cells that have been challenging to maintain in culture. Cells associated with increased cancer risk can also be propagated. Single-cell analyses of matched organoid cultures and native tissues by mass cytometry for 38 markers provide a higher resolution representation of the multiple mammary epithelial cell types in the organoids, and demonstrate that protein expression patterns of the tissue of origin can be preserved in culture. These studies indicate that organoid cultures provide a valuable platform for studies of mammary differentiation, transformation, and breast cancer risk.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-020-15548-7Titel-ID: cdi_doaj_primary_oai_doaj_org_article_9943f96aecad444bafd945d3bf8d8389
- Format
- –
- Schlagworte
- Adult, BRCA1 Protein - genetics, Breast cancer, Breast Neoplasms, Cell culture, Cell Culture Techniques - methods, Cell differentiation, Cell Differentiation - genetics, Cell Differentiation - physiology, Cell Lineage - genetics, Cytometry, Epidermal Growth Factor - pharmacology, Epithelial cells, ErbB Receptors - metabolism, Female, Health risks, Humans, Mammary gland, Mammary Glands, Human - chemistry, Mammary Glands, Human - cytology, Mammary Glands, Human - metabolism, Middle Aged, Organoids, Organoids - chemistry, Organoids - cytology, Organoids - metabolism, p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors, Progenitor cells, Propagation, Single-Cell Analysis, Stem cells, Stem Cells - chemistry, Stem Cells - cytology, Stem Cells - metabolism, Technology, Tissues, Transforming Growth Factor beta - antagonists & inhibitors, Young Adult