Details

Autor(en) / Beteiligte

• Kilens, Stéphanie
• Meistermann, Dimitri
• Moreno, Diego
• Chariau, Caroline
• Gaignerie, Anne
• Reignier, Arnaud
• Lelièvre, Yohann
• Casanova, Miguel
• Vallot, Céline
• Nedellec, Steven
• Flippe, Léa
• Firmin, Julie
• Song, Juan
• Charpentier, Eric
• Lammers, Jenna
• Donnart, Audrey
• Marec, Nadège
• Deb, Wallid
• Bihouée, Audrey
• Le Caignec, Cédric
• Pecqueur, Claire
• Redon, Richard
• Barrière, Paul
• Bourdon, Jérémie
• Pasque, Vincent
• Soumillon, Magali
• Mikkelsen, Tarjei S.
• Rougeulle, Claire
• Fréour, Thomas
• David, Laurent

Titel

Parallel derivation of isogenic human primed and naive induced pluripotent stem cells

Ist Teil von

• Nature communications, 2018-01, Vol.9 (1), p.360-13, Article 360

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Induced pluripotent stem cells (iPSCs) have considerably impacted human developmental biology and regenerative medicine, notably because they circumvent the use of cells of embryonic origin and offer the potential to generate patient-specific pluripotent stem cells. However, conventional reprogramming protocols produce developmentally advanced, or primed, human iPSCs (hiPSCs), restricting their use to post-implantation human development modeling. Hence, there is a need for hiPSCs resembling preimplantation naive epiblast. Here, we develop a method to generate naive hiPSCs directly from somatic cells, using OKMS overexpression and specific culture conditions, further enabling parallel generation of their isogenic primed counterparts. We benchmark naive hiPSCs against human preimplantation epiblast and reveal remarkable concordance in their transcriptome, dependency on mitochondrial respiration and X-chromosome status. Collectively, our results are essential for the understanding of pluripotency regulation throughout preimplantation development and generate new opportunities for disease modeling and regenerative medicine. Derivation of human induced pluripotent stem cells (hiPSCs) produces primed hiPSCs that can in turn be converted to naive hiPSCs. Here, the authors directly reprogram somatic cells to form both naive and primed isogenic hiPSCs and confirm the similarity of naive hiPSCs to their in vivo counterparts.