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Human ARF Specifically Inhibits Epimorphic Regeneration in the Zebrafish Heart
Ist Teil von
Genes, 2020-06, Vol.11 (6), p.666
Ort / Verlag
Switzerland: MDPI AG
Erscheinungsjahr
2020
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
The Alternative Reading Frame (ARF) protein is a tumor suppressor encoded by the
gene in mammals but not lower regenerative vertebrates, and has been previously implicated as a context-sensitive suppressor of regeneration in murine skeletal muscle and humanized ARF-expressing zebrafish fins. This study extends our investigation of the role of ARF in the regeneration of other solid tissues, including the zebrafish heart and the mammalian digit. Heart regeneration after cryoinjury was used to mimic massive myocardial infarction.
gene expression was upregulated during the cardiac regenerative process and slowed the rate of morphological recovery.
specifically impacts cardiomyocytes, neovascularization, and the endothelial-mesenchymal transition, while not affecting epicardial proliferation. This suggests that in the context of regeneration,
is specifically expressed in cells undergoing dedifferentiation. To investigate
as a suppressor of epimorphic regeneration in mammalian systems, we also tested whether the absence of
was permissive for murine digit regeneration, but found that
absence alone was insufficient to significantly alter digit restoration. These findings provide additional evidence that
suppresses epimorphic regeneration, but suggests that modulation of
alone is insufficient to permit regeneration.