Malaria journal, 2022-06, Vol.21 (1), p.1-191, Article 191
2022
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- Autor(en) / Beteiligte
- Titel
- Cellular and antibody response in GMZ2-vaccinated Gabonese volunteers in a controlled human malaria infection trial
- Ist Teil von
- Malaria journal, 2022-06, Vol.21 (1), p.1-191, Article 191
- Ort / Verlag
- London: BioMed Central Ltd
- Erscheinungsjahr
- 2022
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- Abstract Background Antibody and cellular memory responses following vaccination are important measures of immunogenicity. These immune markers were quantified in the framework of a vaccine trial investigating the malaria vaccine candidate GMZ2. Methods Fifty Gabonese adults were vaccinated with two formulations (aluminum Alhydrogel and CAF01) of GMZ2 or a control vaccine (Verorab). Vaccine efficacy was assessed using controlled human malaria infection (CHMI) by direct venous inoculation of 3200 live Plasmodium falciparum sporozoites (PfSPZ Challenge). GMZ2-stimulated T and specific B-cell responses were estimated by flow cytometry before and after vaccination. Additionally, the antibody response against 212 P. falciparum antigens was estimated before CHMI by protein microarray. Results Frequencies of pro- and anti-inflammatory CD4 + T cells stimulated with the vaccine antigen GMZ2 as well as B cell profiles did not change after vaccination. IL-10-producing CD4 + T cells and CD20 + IgG + B cells were increased post-vaccination regardless of the intervention, thus could not be specifically attributed to any malaria vaccine regimen. In contrast, GMZ2-specific antibody response increased after the vaccination, but was not correlated to protection. Antibody responses to several P. falciparum blood and liver stage antigens (MSP1, MSP4, MSP8, PfEMP1, STARP) as well as the breadth of the malaria-specific antibody response were significantly higher in protected study participants. Conclusions In lifelong malaria exposed adults, the main marker of protection against CHMI is a broad antibody pattern recognizing multiple stages of the plasmodial life cycle. Despite vaccination with GMZ2 using a novel formulation, expansion of the GMZ2-stimulated T cells or the GMZ2-specific B cell response was limited, and the vaccine response could not be identified as a marker of protection against malaria. Trial registration PACTR; PACTR201503001038304; Registered 17 February 2015; https://pactr.samrc.ac.za/TrialDisplay.aspx?TrialID=1038
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1475-2875eISSN: 1475-2875DOI: 10.1186/s12936-022-04169-8Titel-ID: cdi_doaj_primary_oai_doaj_org_article_978d148c1f4c454e841b756ef9fb6d02
- Format
- –
- Schlagworte
- Aluminium, Aluminum, Antibodies, Antibody response, Antigens, CD20 antigen, CD4 antigen, Cells, CHMI, Cytokine, Cytokines, Disease control, Flow cytometry, GMZ2, Health aspects, Hepatitis, HIV, Human diseases, Human immunodeficiency virus, Immune system, Immunogenicity, Immunoglobulin G, Immunological memory, Infections, Inflammation, Inoculation, Interleukin 10, Life cycle, Life cycles, Lymphocytes, Lymphocytes B, Lymphocytes T, Malaria, Malaria vaccine, Memory B cells, Microarray, P. falciparum, Parasites, Physiological aspects, Plasmodium falciparum, Protection, Protein arrays, Proteins, Recombinant proteins, Sporozoites, Testing, Vaccination, Vaccine efficacy, Vaccines, Vector-borne diseases, Viral antibodies, Viruses