Membranes (Basel), 2021-08, Vol.11 (8), p.634
2021
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Details
- Autor(en) / Beteiligte
- Titel
- HiLo Based Line Scanning Temporal Focusing Microscopy for High-Speed, Deep Tissue Imaging
- Ist Teil von
- Membranes (Basel), 2021-08, Vol.11 (8), p.634
- Ort / Verlag
- Basel: MDPI AG
- Erscheinungsjahr
- 2021
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- High-speed, optical-sectioning imaging is highly desired in biomedical studies, as most bio-structures and bio-dynamics are in three-dimensions. Compared to point-scanning techniques, line scanning temporal focusing microscopy (LSTFM) is a promising method that can achieve high temporal resolution while maintaining a deep penetration depth. However, the contrast and axial confinement would still be deteriorated in scattering tissue imaging. Here, we propose a HiLo-based LSTFM, utilizing structured illumination to inhibit the fluorescence background and, thus, enhance the image contrast and axial confinement in deep imaging. We demonstrate the superiority of our method by performing volumetric imaging of neurons and dynamical imaging of microglia in mouse brains in vivo.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2077-0375eISSN: 2077-0375DOI: 10.3390/membranes11080634Titel-ID: cdi_doaj_primary_oai_doaj_org_article_957fe4f072d54e73802024f2a4ede5f6
- Format
- –
- Schlagworte
- axial confinement enhancement, Biomedical materials, Cameras, Confinement, contrast enhancement, deep tissue imaging, Dynamic structural analysis, Fluorescence, High speed, HiLo microscopy, Image contrast, Image enhancement, Lasers, line scanning temporal focusing microscopy, Microglia, Microscopy, Normal distribution, Optics, Penetration depth, Scanners, Scanning, Sectioning, Temporal resolution