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Efficacy of single and combined applications of entomopathogenic fungi and nematodes against the pupae of colorado potato beetle (Leptinotarsa decemlineata [Say]), (Coleoptera: Chrysomelidae)
Ist Teil von
Egyptian journal of biological pest control, 2022-06, Vol.32 (1), p.1-6, Article 81
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2022
Quelle
Electronic Journals Library
Beschreibungen/Notizen
Background
The lethal effects of different entomopathogenic fungi (EPF) and nematodes (EPN) on the pupae of the Colorado Potato Beetle,
Leptinotarsa decemlineata
(Say) (Coleoptera: Chrysomelidae) were investigated in a two-step study when used separately or together. In the first step, Nostalgist (
Beauveria bassiana
strain Bb-1, Agrobest Co.),
Steinernema feltiae
(Filipjev) (Rhabditida: Steinernematidae),
Heterorhabditis bacteriophora
Poinar, 1976 (Rhabditida: Heterorhabditidae) (Bioglobal Co.), 2 Turkish isolates of
Beauveria bassiana
BIM-001 and BY2 were applied separately to the pupal stage of pest in the soil. In the experiments, 3 different concentrations (150, 200, and 250 million infective juveniles (IJs)/100 l water) of EPN and a single concentration (1x10
8
spores/ml for and maximum recommended field dose 1 × 10
8
CFU/ml) of EPF were used. In the second step, BIM-001 and BY2 isolates of
B. bassiana
were combined with both EPN species;
S. feltiae
+
B
.
bassiana
BIM-001,
S. feltiae
+
B
.
bassiana
BY2,
H. bacteriophora
+
B
.
bassiana
BIM-001,
H. bacteriophora
+
B
.
bassiana
BY2 were applied to pupae. At this stage, 150 million IJs/100 l water which was the maximum recommended field concentration for EPN and 1 × 10
8
spores/ml for local EPF isolates were used.
Results
According to the study results, pupae deaths were significantly higher in Nostalgist (74%) than those of
B
.
bassiana
BIM-001,
B
.
bassiana
BY2,
S
.
feltiae
, and
H
.
bacteriophora
(
P
<0.05). While both EPN caused higher mortality than
B
.
bassiana
BY2,
B
.
bassiana
BIM-001 had the highest mortality rates. The combination of
S. feltiae
+
B. bassiana
BIM-001 at the highest mortality rate (80%) was found statistically different and significant from other EPF + EPN treatments on pupae. Moreover, there were non-statistical differences between the other EPF and EPN combinations.
Conclusion
It was found that the combined use of EPF and EPN was more effective on the pupae of
L
.
decemlineata
than a single application. According to the results of the study, it may be promising to use EPN and EPF together for the control of the Colorado Potato Beetle pupae.