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Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR
Ist Teil von
Cancers, 2021-01, Vol.13 (1), p.150
Ort / Verlag
Switzerland: MDPI AG
Erscheinungsjahr
2021
Link zum Volltext
Quelle
EZB Free E-Journals
Beschreibungen/Notizen
mRNA RT-qPCR is shown to be a very sensitive technique to detect minimal residual disease (MRD) in patients with neuroblastoma. Multiple mRNA markers are known to detect heterogeneous neuroblastoma cells in bone marrow (BM) or blood from patients. However, the limited volumes of BM and blood available can hamper the detection of multiple markers. To make optimal use of these samples, we developed a multiplex RT-qPCR for the detection of MRD in neuroblastoma.
and
were tested as single markers. The adrenergic markers
,
,
and
and mesenchymal markers
,
and
were tested in multiplex. Using control blood and BM, we established new thresholds for positivity. Comparison of multiplex and singleplex RT-qPCR results from 21 blood and 24 BM samples from neuroblastoma patients demonstrated a comparable sensitivity. With this multiplex RT-qPCR, we are able to test seven different neuroblastoma mRNA markers, which overcomes tumor heterogeneity and improves sensitivity of MRD detection, even in those samples of low RNA quantity. With resources and time being saved, reduction in sample volume and consumables can assist in the introduction of MRD by RT-qPCR into clinical practice.