Details

Autor(en) / Beteiligte

• Huber, Madeleine
• Faure, Guilhem
• Laass, Sebastian
• Kolbe, Esther
• Seitz, Kristina
• Wehrheim, Christina
• Wolf, Yuri I
• Koonin, Eugene V
• Soppa, Jörg

Titel

Translational coupling via termination-reinitiation in archaea and bacteria

Ist Teil von

• Nature communications, 2019-09, Vol.10 (1), p.4006-11, Article 4006

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• The genomes of many prokaryotes contain substantial fractions of gene pairs with overlapping stop and start codons (ATGA or TGATG). A potential benefit of overlapping gene pairs is translational coupling. In 720 genomes of archaea and bacteria representing all major phyla, we identify substantial, albeit highly variable, fractions of co-directed overlapping gene pairs. Various patterns are observed for the utilization of the SD motif for de novo initiation at upstream genes versus reinitiation at overlapping gene pairs. We experimentally test the predicted coupling in 9 gene pairs from the archaeon Haloferax volcanii and 5 gene pairs from the bacterium Escherichia coli. In 13 of 14 cases, translation of both genes is strictly coupled. Mutational analysis of SD motifs located upstream of the downstream genes indicate that the contribution of the SD to translational coupling widely varies from gene to gene. The nearly universal, abundant occurrence of overlapping gene pairs suggests that tight translational coupling is widespread in archaea and bacteria.