Genome Biology, 2021-06, Vol.22 (1), p.171-171, Article 171
2021
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- Autor(en) / Beteiligte
- Titel
- Dynamics of alternative splicing during somatic cell reprogramming reveals functions for RNA-binding proteins CPSF3, hnRNP UL1, and TIA1
- Ist Teil von
- Genome Biology, 2021-06, Vol.22 (1), p.171-171, Article 171
- Ort / Verlag
- England: BioMed Central
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Somatic cell reprogramming is the process that allows differentiated cells to revert to a pluripotent state. In contrast to the extensively studied rewiring of epigenetic and transcriptional programs required for reprogramming, the dynamics of post-transcriptional changes and their associated regulatory mechanisms remain poorly understood. Here we study the dynamics of alternative splicing changes occurring during efficient reprogramming of mouse B cells into induced pluripotent stem (iPS) cells and compare them to those occurring during reprogramming of mouse embryonic fibroblasts. We observe a significant overlap between alternative splicing changes detected in the two reprogramming systems, which are generally uncoupled from changes in transcriptional levels. Correlation between gene expression of potential regulators and specific clusters of alternative splicing changes enables the identification and subsequent validation of CPSF3 and hnRNP UL1 as facilitators, and TIA1 as repressor of mouse embryonic fibroblasts reprogramming. We further find that these RNA-binding proteins control partially overlapping programs of splicing regulation, involving genes relevant for developmental and morphogenetic processes. Our results reveal common programs of splicing regulation during reprogramming of different cell types and identify three novel regulators of this process and their targets.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1474-760X, 1474-7596eISSN: 1474-760XDOI: 10.1186/s13059-021-02372-5Titel-ID: cdi_doaj_primary_oai_doaj_org_article_928f71eebddd447f9050eb912dcd5992
- Format
- –
- Schlagworte
- Alternative splicing, Alternative Splicing - genetics, Animals, B-Lymphocytes - metabolism, CCAAT-Enhancer-Binding Proteins - metabolism, Cell differentiation, Cellular Reprogramming - genetics, Cleavage And Polyadenylation Specificity Factor - metabolism, CPSF3, Datasets, Efficiency, Embryo fibroblasts, Embryo, Mammalian - cytology, Epigenetics, Fibroblasts - metabolism, Gene expression, Gene Expression Regulation, Developmental, Gene regulation, Heterogeneous-Nuclear Ribonucleoproteins - metabolism, hnRNP UL1, Lymphocytes B, Mice, Pluripotency, Post-transcription, Proteins, RNA-binding protein, Somatic cell reprogramming, T-Cell Intracellular Antigen-1 - metabolism, TIA1, Transcription factors