Details

Autor(en) / Beteiligte

• Mansouri, Maysam
• Bellon-Echeverria, Itxaso
• Rizk, Aurélien
• Ehsaei, Zahra
• Cianciolo Cosentino, Chiara
• Silva, Catarina S.
• Xie, Ye
• Boyce, Frederick M.
• Davis, M. Wayne
• Neuhauss, Stephan C. F.
• Taylor, Verdon
• Ballmer-Hofer, Kurt
• Berger, Imre
• Berger, Philipp

Titel

Highly efficient baculovirus-mediated multigene delivery in primary cells

Ist Teil von

• Nature communications, 2016-05, Vol.7 (1), p.11529-11529, Article 11529

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Multigene delivery and subsequent cellular expression is emerging as a key technology required in diverse research fields including, synthetic and structural biology, cellular reprogramming and functional pharmaceutical screening. Current viral delivery systems such as retro- and adenoviruses suffer from limited DNA cargo capacity, thus impeding unrestricted multigene expression. We developed MultiPrime, a modular, non-cytotoxic, non-integrating, baculovirus-based vector system expediting highly efficient transient multigene expression from a variety of promoters. MultiPrime viruses efficiently transduce a wide range of cell types, including non-dividing primary neurons and induced-pluripotent stem cells (iPS). We show that MultiPrime can be used for reprogramming, and for genome editing and engineering by CRISPR/Cas9. Moreover, we implemented dual-host-specific cassettes enabling multiprotein expression in insect and mammalian cells using a single reagent. Our experiments establish MultiPrime as a powerful and highly efficient tool, to deliver multiple genes for a wide range of applications in primary and established mammalian cells. Current viral gene delivery systems are limited in the amount of foreign DNA they can deliver to cells. Here the authors develop MultiPrime, a baculovirus-based vector system capable of multigene delivery into a wide variety of cells, and use Multiprime for genome engineering by CRISPR/Cas9.