Marine drugs, 2013-10, Vol.11 (11), p.4193-4212
2013
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Details
- Autor(en) / Beteiligte
- Titel
- Anti-diabetic effect of balanced deep-sea water and its mode of action in high-fat diet induced diabetic mice
- Ist Teil von
- Marine drugs, 2013-10, Vol.11 (11), p.4193-4212
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2013
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- In this study, we investigated the effects of balanced deep-sea water (BDSW) on hyperglycemia and glucose intolerance in high-fat diet (HFD)-induced diabetic C57BL/6J mice. BDSW was prepared by mixing deep-sea water (DSW) mineral extracts and desalinated water to give a final hardness of 500-2000. Mice given an HFD with BDSW showed lowered fasting plasma glucose levels compared to HFD-fed mice. Oral and intraperitoneal glucose tolerance tests showed that BDSW improves impaired glucose tolerance in HFD-fed mice. Histopathological evaluation of the pancreas showed that BDSW recovers the size of the pancreatic islets of Langerhans, and increases the secretion of insulin and glucagon in HFD-fed mice. Quantitative reverse transcription polymerase chain reaction results revealed that the expression of hepatic genes involved in glucogenesis, glycogenolysis and glucose oxidation were suppressed, while those in glucose uptake, β-oxidation, and glucose oxidation in muscle were increased in mice fed HFD with BDSW. BDSW increased AMP-dependent kinase (AMPK) phosphorylation in 3T3-L1 pre- and mature adipocytes and improved impaired AMPK phosphorylation in the muscles and livers of HFD-induced diabetic mice. BDSW stimulated phosphoinositol-3-kinase and AMPK pathway-mediated glucose uptake in 3T3-L1 adipocytes. Taken together, these results suggest that BDSW has potential as an anti-diabetic agent, given its ability to suppress hyperglycemia and improve glucose intolerance by increasing glucose uptake.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1660-3397eISSN: 1660-3397DOI: 10.3390/md11114193Titel-ID: cdi_doaj_primary_oai_doaj_org_article_916e699e31c24d4a91b79eb4b3e70acd
- Format
- –
- Schlagworte
- 3T3-L1 Cells, Adipocytes - drug effects, Adipocytes - metabolism, Animals, Blood Glucose - drug effects, Cell Line, deep-sea water, diabetes, Diabetes Mellitus - metabolism, Diet, High-Fat - adverse effects, Glucagon - metabolism, Glucose - metabolism, glucose tolerance, Glucose Tolerance Test - methods, high-fat diet, hyperglycemia, Hypoglycemic Agents - pharmacology, Insulin - metabolism, Islets of Langerhans - drug effects, Islets of Langerhans - metabolism, Male, Mice, Mice, Inbred C57BL, Phosphorylation - drug effects, Seawater, Signal Transduction - drug effects, Water - metabolism, Water - pharmacology