Details

Autor(en) / Beteiligte

• Deleye, Lieselot
• Tilleman, Laurentijn
• Vander Plaetsen, Ann-Sophie
• Cornelis, Senne
• Deforce, Dieter
• Van Nieuwerburgh, Filip

Titel

Performance of four modern whole genome amplification methods for copy number variant detection in single cells

Ist Teil von

• Scientific reports, 2017-06, Vol.7 (1), p.3422-9, Article 3422

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Whole genome amplification (WGA) has become an invaluable tool to perform copy number variation (CNV) detection in single, or a limited number of cells. Unfortunately, current WGA methods introduce representation bias that limits the detection of small CNVs. New WGA methods have been introduced that might have the potential to reduce this bias. We compared the performance of PicoPLEX DNA-Seq (Picoseq), DOPlify, REPLI-g and Ampli-1 WGA for aneuploidy screening and copy number analysis using shallow whole genome massively parallel sequencing (MPS), starting from single or a limited number of cells. Although the four WGA methods perform differently, they are all suited for this application.