Details

Autor(en) / Beteiligte

• Wang, Zhaoqi
• Jung, Soontag
• Yeo, Daseul
• Woo, Seoyoung
• Seo, Yeeun
• Hossain, Md. Iqbal
• Kwon, Hyojin
• Jeong, Myeong-In
• Choi, Changsun

Titel

Combination of paper membrane-based filtration and ultrafiltration to enhance the detection of MNV, HAV, and HCoV from soil-rich post-washing water

Ist Teil von

• iScience, 2022-12, Vol.25 (12), p.105640-105640, Article 105640

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Risk-assessing and controlling virus transmission from soil-rich post-washing water (PWW) are crucial during harvesting raw vegetables. However, viruses are normally difficult to concentrate because of their low concentrations and complex backgrounds. Here, ultrafiltration (UF), virus adsorption-elution (VIRADEL), and optimized paper filtration-coupled ultrafiltration (PFC-UF) methods were employed to evaluate the recovery of non-enveloped murine norovirus (MNV-1), hepatitis A virus (HAV), and enveloped human coronavirus 229E (HCoV-229E) from soil-rich PWW. Among the three methods, PFC-UF outperformed the other methods in the recovery of viruses from PWW with soil content. Under the highest soil condition with virus seeded at a titer of 102 plaque-forming unit (PFU) or TCID50, the PFC-UF method exhibited an exceedingly consistent recovery rate of 78.8 ± 13.3 (MNV-1) and 44.4 ± 25.2% (HAV). However, the recovery of enveloped HCoV-229E was inferior to non-enveloped viruses. Overall, PFC-UF provided a reliable method for recovering viruses in soil-rich PWW. [Display omitted] •PFC-UF outperformed others in the recovery of viruses from PWW with soil content•MNV-1 and HAV were recovered 78.8% and 44.5% from PWW with the highest soil content•Enveloped and non-enveloped viruses differed in their recovery efficiencies•PFC-UF presented a feasible approach to detecting the viruses in PWW Virology; Membrane system