Details

Autor(en) / Beteiligte

• Witkowska, Agata
• Heinz, Leonard P.
• Grubmüller, Helmut
• Jahn, Reinhard

Titel

Tight docking of membranes before fusion represents a metastable state with unique properties

Ist Teil von

• Nature communications, 2021-06, Vol.12 (1), p.3606-3606, Article 3606

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Abstract Membrane fusion is fundamental to biological processes as diverse as membrane trafficking or viral infection. Proteins catalyzing membrane fusion need to overcome energy barriers to induce intermediate steps in which the integrity of bilayers is lost. Here, we investigate the structural features of tightly docked intermediates preceding hemifusion. Using lipid vesicles in which progression to hemifusion is arrested, we show that the metastable intermediate does not require but is enhanced by divalent cations and is characterized by the absence of proteins and local membrane thickening. Molecular dynamics simulations reveal that thickening is due to profound lipid rearrangements induced by dehydration of the membrane surface.