Details

Autor(en) / Beteiligte

• McCormick, Rachel A
• Ralbovsky, Nicole M
• Gilbraith, William
• Smith, Joseph P
• Booksh, Karl S

Titel

Analyzing atomic force microscopy images of virus-like particles by expectation-maximization

Ist Teil von

• npj vaccines, 2024-06, Vol.9 (1), p.112-11, Article 112

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2024

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Abstract Analysis of virus-like particles (VLPs) is an essential task in optimizing their implementation as vaccine antigens for virus-initiated diseases. Interrogating VLP collections for elasticity by probing with a rigid atomic force microscopy (AFM) tip is a potential method for determining VLP morphological changes. During VLP morphological change, it is not expected that all VLPs would be in the same state. This leads to the open question of whether VLPs may change in a continuous or stepwise fashion. For continuous change, the statistical distribution of observed VLP properties would be expected as a single distribution, while stepwise change would lead to a multimodal distribution of properties. This study presents the application of a Gaussian mixture model (GMM), fit by the Expectation-Maximization (EM) algorithm, to identify different states of VLP morphological change observed by AFM imaging.