Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Core Fucosylation of Intestinal Epithelial Cells Protects Against Salmonella Typhi Infection via Up-Regulating the Biological Antagonism of Intestinal Microbiota
Ist Teil von
Frontiers in microbiology, 2020-05, Vol.11, p.1097-1097
Ort / Verlag
Frontiers Media S.A
Erscheinungsjahr
2020
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
The fucosylated carbohydrate moieties on intestinal epithelial cells (IECs) are involved in the creation of an environmental niche for commensal and pathogenic bacteria. Core fucosylation catalyzed by fucosyltransferase 8 (Fut8) is the major fucosylation pattern on the
N
-glycans of the surface glycoproteins on IECs, however, the role of IECs core fucosylation during infection remains unclear. This study was conducted to investigate the interaction between IECs core fucosylation and gut microbiota, and the effects of this interaction on protecting
Salmonella enterica
subsp.
enterica
serovar Typhi (
S.
Typhi) infection. Firstly, the
Fut8
+/+
and
Fut8
+/–
mice were infected with
S.
Typhi. The level of IECs core fucosylation and protein expression of intestinal mucosa were then detected by LCA blot and Western blot, respectively. The gut microbiota of
Fut8
+/+
and
Fut8
+/–
mice before and after
S.
Typhi infection was assessed by 16S rRNA sequencing. Our results showed that core fucosylation was ubiquitous expressed on the intestinal mucosa of mice and had significant effects on their gut microbiota. Fut8
+/–
mice was more susceptive to
S.
Typhi infection than Fut8
+/+
mice. Interestingly, infection of
S.
Typhi upregulated the core fucosylation level of IECs and increased the abundances of beneficial microorganisms such as
Lactobacillus
and
Akkermansia
spp. Further
in vitro
and
in vivo
studies demonstrated that Wnt/β-catenin signaling pathway mediated the elevation of IECs core fucosylation level upon infection of
S.
Typhi. Taken together, our data in this study revealed that the IECs core fucosylation plays an important role in protecting against
S.
Typhi infection via up-regulating the biological antagonism of intestinal microbiota.