Details

Autor(en) / Beteiligte

• Roman-Canal, Berta
• Tarragona, Jordi
• Moiola, Cristian Pablo
• Gatius, Sònia
• Bonnin, Sarah
• Ruiz-Miró, Maria
• Sierra, José Enrique
• Rufas, Maria
• González, Esperanza
• Porcel, José M
• Gil-Moreno, Antonio
• Falcón-Pérez, Juan M
• Ponomarenko, Julia
• Matias-Guiu, Xavier
• Colas, Eva

Titel

EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer

Ist Teil von

• Journal of translational medicine, 2019-06, Vol.17 (1), p.208-208, Article 208

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2019

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.