Details

Autor(en) / Beteiligte

• Osuna-Amarillas, Pablo Sergio
• Rouzaud-Sandez, Ofelia
• Higuera-Barraza, Odilia Azucena
• Arias-Moscoso, Joe Luis
• López-Mata, Marco Antonio
• Campos-García, Julio César
• Valdez-Melchor, Ramón Gertrudis

Titel

Hydrophobic interaction chromatography as separation method of alkaline proteases from viscera of Scomberomorus sierra

Ist Teil von

• TIP. Revista especializada en ciencias químico-biológicas, 2019-07, Vol.22, p.1-10

Ort / Verlag

Universidad Nacional Autónoma de México, Facultad de Estudios Superiores, Plantel Zaragoza

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Abstract This study focused on recovering alkaline proteases from the viscera of Scomberomorus sierra through hydrophobic interaction chromatography. Three alkaline proteases were partially separated using this chromatographic technique; two of them, with molecular weights of 19 and 31 kDa, were identified as trypsin-like enzymes according to inhibition assays. The 31 kDa alkaline protease, the only isolated enzyme, was purified under following chromatographic conditions: ammonium sulfate 13% (w/v) and ethylene glycol 27% (w/v); this enzyme showed maximum activity at pH 9 - 10 and 50 - 60 °C and was strongly inhibited by soybean trypsin inhibitor (SBTI) and porcine trypsin inhibitor (TPI). A third alkaline protease with molecular weight of 20 kDa was partially separated and inhibited by tosyl phenylalanyl chloromethyl ketone (TPCK), showing optimum activity at pH 9 - 11 and 60 °C. These results show that the viscera of Scomberomorus sierra may be useful as source of proteases.