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In-depth investigation of microRNA-mediated cross-kingdom regulation between Asian honey bee and microsporidian
Ist Teil von
Frontiers in microbiology, 2022-09, Vol.13, p.1003294-1003294
Ort / Verlag
Frontiers Media S.A
Erscheinungsjahr
2022
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Asian honey bee
Apis cerana
is the original host for
Nosema ceranae
, a unicellular fungal parasite that causes bee nosemosis throughout the world. Currently, interaction between
A. cerana
and
N. ceranae
is largely unknown. Our group previously prepared
A. c. cerana
workers’ midguts at 7 days post inoculation (dpi) and 10 dpi with
N. ceranae
spores as well as corresponding un-inoculated workers’ midguts, followed by cDNA library construction and a combination of RNAs-seq and small RNA-seq. Meanwhile, we previously prepared clean spores of
N. ceranae
, which were then subjected to cDNA library construction and deep sequencing. Here, based on the gained high-quality transcriptome datasets,
N. ceranae
differentially expressed mRNAs (DEmiRNAs) targeted by host DEmiRNAs, and
A. c. cerana
DEmRNAs targeted by microsporidian DEmiRNAs were deeply investigated, with a focus on targets involved in
N. ceranae
glycolysis/glyconeogenesis as well as virulence factors, and
A. c. cerana
energy metabolism and immune response. In
A. c. cerana
worker’s midguts at 7 (10) dpi (days post inoculation), eight (seven) up-regulated and six (two) down-regulated miRNAs were observed to target 97 (44) down-regulated and 60 (15) up-regulated
N. ceranae
mRNAs, respectively. Additionally, two up-regulated miRNAs (miR-60-y and miR-676-y) in host midgut at 7 dpi could target genes engaged in
N. ceranae
spore wall protein and glycolysis/gluconeogenesis, indicating potential host miRNA-mediated regulation of microsporidian virulence factor and energy metabolism. Meanwhile, in
N. ceranae
at 7 (10) dpi, 121 (110) up-regulated and 112 (104) down-regulated miRNAs were found to, respectively, target 343 (247) down-regulated and 138 (110) down-regulated mRNAs in
A. c. cerana
workers’ midguts. These targets in host were relevant to several crucial cellular and humoral immune pathways, such as phagasome, endocytosis, lysosomes, regulation of autophagy, and Jak–STAT signaling pathway, indicative of the involvement of
N. ceranae
DEmiRNAs in regulating these cellular and humoral immune pathways. In addition,
N. ceranae
miR-21-x was up-regulated at 7 dpi and had a target relative to oxidative phosphorylation, suggesting that miR-21-x may be used as a weapon to modulate this pivotal energy metabolism pathway. Furthermore, potential targeting relationships between two pairs of host DEmiRNAs-microsporidian DEmRNAs and two pairs of microsporidian DEmiRNAs-host DEmRNAs were validated using RT-qPCR. Our findings not only lay a foundation for exploring the molecular mechanism underlying cross-kingdom regulation between
A. c. cerana
workers and
N. ceranae
, but also offer valuable insights into Asian honey bee-microsporidian interaction.