Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Protective effects of lutein against phone screen light-induced damage on 3D bioprinted retinal pigment epithelium monolayers
Ist Teil von
Journal of functional foods, 2024-05, Vol.116, p.106216, Article 106216
Ort / Verlag
Elsevier Ltd
Erscheinungsjahr
2024
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
[Display omitted]
•The first 3D RPE monolayer was used for studying dietary antioxidants.•Phone screen light reduced cell viability and increased oxidative stress levels.•Lutein promoted cell survival by reducing oxidative stress.•Lutein improved barrier and phagocytosis functions of light-damaged RPE monolayer.•The highest protective effects were observed with 0.5 µM lutein.
Cumulative light exposure, including electronic device radiation, may induce retinal pigment epithelium (RPE)-related retinal diseases, potentially mitigated by lutein. However, there is limited 3D RPE monolayer model to study the effect of phytochemicals on their activity and action mechanisms in vitro. Here, we present a method using a monolayer RPE tissue model on a 3D-printed polycaprolactone scaffold. Six-hour of phone screen light increased reactive oxygen species (ROS) in RPE cells, leading to oxidative stress, cytotoxicity, and inhibited metabolism. Pre-treating RPE cells (24 h) with 0.5 µM lutein reduced ROS levels and maintained viability under the same light exposure. Additionally, lutein demonstrated protective effects on crucial RPE functions, including phagocytosis and the retinal-blood barrier. This study underscores the importance of optimal lutein content in shielding RPE from phone screen light damage, and our model holds promise for evaluating the efficacy of antioxidants against light radiation.