Details

Autor(en) / Beteiligte

• Xie, Zhenyu
• Niu, Liaoran
• Zheng, Gaozan
• Du, Kunli
• Dai, Songchen
• Li, Ruikai
• Dan, Hanjun
• Duan, Lili
• Wu, Hongze
• Ren, Guangming
• Dou, Xinyu
• Feng, Fan
• Zhang, Jian
• Zheng, Jianyong

Titel

Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment

Ist Teil von

• Cell & bioscience, 2023-11, Vol.13 (1), p.1-217, Article 217

Ort / Verlag

London: BioMed Central Ltd

Erscheinungsjahr

2023

Quelle

Springer Online Journals

Beschreibungen/Notizen

• The role of mast cells (MCs) in colorectal cancer (CRC) remains unclear, and a comprehensive single-cell study on CRC MCs has not been conducted. This study used a multi-omics approach, integrating single-cell sequencing, spatial transcriptomics, and bulk tissue sequencing data to investigate the heterogeneity and impact of MCs in CRC. Five MC signature genes (TPSAB1, TPSB2, CPA3, HPGDS, and MS4A2) were identified, and their average expression was used as a marker of MCs. The MC density was found to be lower in CRC compared to normal tissue, but MCs in CRC demonstrated distinct activation features. Activated MCs were defined by high expression of receptors and MC mediators, while resting MCs had low expression. Most genes, including the five MC signature genes, were expressed at higher levels in activated MCs. The MC signature was linked to a better prognosis in both CRC and pan-cancer patient cohorts. Elevated KITLG expression was observed in fibroblasts and endothelial cells in CRC samples compared to normal tissue, and co-localization of MCs with these cell types was revealed by spatial transcriptome analysis. In conclusion, this study finds decreased MC density in CRC compared to normal tissue, but highlights a shift in MC phenotype from CMA1.sup.high resting cells to activated TPSAB1.sup.high, CPA3.sup.high, and KIT.sup.high cells. The elevated KITLG expression in the tumor microenvironment's fibroblasts and endothelial cells may activate MCs through the KITLG-KIT axis, potentially suppressing tumor progression.